High insecticide resistance mediated by different mechanisms in Culex quinquefasciatus populations from the city of Yaoundé, Cameroon

Talipouo, Abdou, Mavridis, Konstantinos, Nchoutpouen, Elysée, Djiappi-Tchamen, Borel, A. Fotakis, Emmanouil, Kopya, Edmond, Bamou, Roland, Kekeunou, Sévilor, Awono-Ambene, Parfait, Balabanidou, Vasileia, Balaska, Sofia, Wondji, Charles ORCID: https://orcid.org/0000-0003-0791-3673, Vontas, John and Nkondjio, Christophe (2021) 'High insecticide resistance mediated by different mechanisms in Culex quinquefasciatus populations from the city of Yaoundé, Cameroon'. Scientific Reports, Vol 11, p. 7322.

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Abstract

Culex mosquitoes particularly Culex quinquefasciatus are important arboviral and filariasis vectors, however despite this important epidemiological role, there is still a paucity of data on their bionomics. The present study was undertaken to assess the insecticide resistance status of Cx. quinquefasciatus populations from four districts of Yaoundé (Cameroon).
All Culex quinquefasciatus populations except one displayed high resistance to bendiocarb and malathion with mortalities ranging from 0-89% while high resistance intensity against both permethrin and deltamethrin was recorded. Molecular analyses revealed high frequencies of the ACE-1 G119S mutation (ranging from 0-33%) and kdr L1014F allele (ranging from 55-74%) in all Cx. quinquefasciatus populations. Significant overexpression was detected for cytochrome P450s genes CYP6AA7 and CYP6Z10, as well as for Esterase A and Esterase B genes. The total cuticular hydrocarbon content, a proxy of cuticular resistance, was significantly increased (compared to the S-lab strain) in one population.
The study confirms strong insecticide resistance mediated by different mechanisms in Cx. quinquefasciatus populations from the city of Yaoundé. The expansion of insecticide resistance in Culex populations could affect the effectiveness of current vector control measures and stress the need for the implementation of integrated vector control strategies in urban settings.
Keywords: Culex quinquefasciatus, insecticide resistance, metabolic resistance, kdr, Yaoundé

Item Type:	Article
Subjects:	QX Parasitology > Insects. Other Parasites > QX 510 Mosquitoes QX Parasitology > Insects. Other Parasites > QX 530 Culex QX Parasitology > Insects. Other Parasites > QX 600 Insect control. Tick control WA Public Health > Preventive Medicine > WA 240 Disinfection. Disinfestation. Pesticides (including diseases caused by) WC Communicable Diseases > Tropical and Parasitic Diseases > WC 765 Prevention and control
Faculty: Department:	Biological Sciences > Vector Biology Department
Digital Object Identifer (DOI):	https://doi.org/10.1038/s41598-021-86850-7
Depositing User:	Mel Finley
Date Deposited:	13 May 2021 16:16
Last Modified:	13 May 2021 16:16
URI:	https://archive.lstmed.ac.uk/id/eprint/17801

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