Tools
Tools
Enne, Virve I, Aydin, Alp, Baldan, Rossella, Owen, Dewi R, Richardson, Hollian, Ricciardi, Federico, Russell, Charlotte, Nomamiukor-Ikeji, Brenda O., Swart, Ann Marie, High, Juliet, Colles, Antony, Barber, Julie A, Gant, Vanya, Livermore, David M and O’Grady, Justin (2022) 'Multicentre evaluation of two multiplex PCR platforms for the rapid microbiologicalinvestigation of nosocomial pneumonia in UK ICUs: the INHALE WP1 study'. Thorax, Vol 77, Issue 12, pp. 1220-1228.
|
Text
thoraxjnl-2021-216990.R2_Proof_hi.pdf - Accepted Version Available under License Creative Commons Attribution Non-commercial. Download (1MB) | Preview |
Abstract
Background
Culture-based microbiological investigation of hospital-acquired or ventilator-associated pneumonia (HAP or VAP) is insensitive, with aetiological agents often unidentified. This can lead to excess antimicrobial treatment of patients with susceptible pathogens, while those with resistant bacteria are treated inadequately for prolonged periods. Using PCR to seek pathogens and their resistance genes directly from clinical samples may improve therapy and stewardship.
Methods
Surplus routine lower respiratory tract samples were collected from intensive care unit patients about to receive new or changed antibiotics for hospital-onset lower respiratory tract infections at 15 UK hospitals. Testing was performed using the BioFire FilmArray Pneumonia Panel (bioMérieux) and Unyvero Pneumonia Panel (Curetis). Concordance analysis compared machine and routine microbiology results, while Bayesian latent class (BLC) analysis estimated the sensitivity and specificity of each test, incorporating information from both PCR panels and routine microbiology.
Findings
In 652 eligible samples; PCR identified pathogens in considerably more samples compared with routine microbiology: 60.4% and 74.2% for Unyvero and FilmArray respectively vs 44.2% by routine microbiology. PCR tests also detected more pathogens per sample than routine microbiology. For common HAP/VAP pathogens, FilmArray had sensitivity of 91.7%–100.0% and specificity of 87.5%–99.5%; Unyvero had sensitivity of 50.0%–100.0%%, and specificity of 89.4%–99.0%. BLC analysis indicated that, compared with PCR, routine microbiology had low sensitivity, ranging from 27.0% to 69.4%.
Interpretation
Conventional and BLC analysis demonstrated that both platforms performed similarly and were considerably more sensitive than routine microbiology, detecting potential pathogens in patient samples reported as culture negative. The increased sensitivity of detection realised by PCR offers potential for improved antimicrobial prescribing.
| Item Type: | Article |
|---|---|
| Subjects: | QW Microbiology and Immunology > QW 4 General works. Classify here works on microbiology as a whole. QY Clinical Pathology > QY 4 General works WC Communicable Diseases > Infection. Bacterial Infections > Bacterial Infections > WC 202 Pneumonia (General or not elsewhere classified) WF Respiratory System > WF 20 Research (General) |
| Faculty: Department: | Clinical Sciences & International Health > Clinical Sciences Department |
| Digital Object Identifer (DOI): | https://doi.org/10.1136/thoraxjnl-2021-216990 |
| Depositing User: | Marie Hatton |
| Date Deposited: | 31 Jan 2022 11:30 |
| Last Modified: | 02 Feb 2023 09:04 |
| URI: | https://archive.lstmed.ac.uk/id/eprint/19680 |
Statistics
Actions (login required)
 |
Edit Item |