Intracellular survival of Streptococcus pneumoniae in human alveolar macrophages is augmented with HIV infection

Nyazika, Tinashe, Sibale, Lusako, Phiri, Joseph, De Ste Croix, Megan, Jasiunaite, Zydrune, Mkandawire, Christopher, Malamba, Rose, Kankwatira, Anstead, Manduwa, Miriam, Ferreira, Daniela ORCID: https://orcid.org/0000-0002-0594-0902, Nyirenda, Tonney S., Oggioni, Marco R., Mwandumba, Henry ORCID: https://orcid.org/0000-0003-4470-3608 and Jambo, Kondwani ORCID: https://orcid.org/0000-0002-3195-2210 (2022) 'Intracellular survival of Streptococcus pneumoniae in human alveolar macrophages is augmented with HIV infection'. Frontiers in Immunology, Vol 13, e992659.

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Abstract

People Living with HIV (PLHIV) are at an increased risk of pneumococcal pneumonia than HIV-uninfected adults, but the reasons for this are still not well understood. We investigated whether alveolar macrophages (AM) mediated control of pneumococcal infection is impaired in PLHIV compared to HIV-uninfected adults. We assessed anti-bactericidal activity against Streptococcus pneumoniae of primary human AM obtained from PLHIV and HIV-uninfected adults. We found that pneumococcus survived intracellularly in AMs at least 24 hours post ex vivo infection, and this was more frequent in PLHIV than HIV-uninfected adults. Corroborating these findings, in vivo evidence showed that PLHIV had a higher propensity for harboring S. pneumoniae within their AMs than HIV-uninfected adults. Moreover, bacterial intracellular survival in AMs was associated with extracellular propagation of pneumococcal infection. Our data suggest that failure of AMs to eliminate S. pneumoniae intracellularly could contribute to the increased risk of pneumococcal pneumonia in PLHIV.

Item Type:	Article
Subjects:	QU Biochemistry > Cells and Genetics > QU 300 General works QW Microbiology and Immunology > QW 50 Bacteria (General). Bacteriology. Archaea WC Communicable Diseases > Infection. Bacterial Infections > Bacterial Infections > WC 210 Streptococcal infections (General or not elsewhere classified) WC Communicable Diseases > Virus Diseases > Acquired Immunodeficiency Syndrome. HIV Infections > WC 503 Acquired immunodeficiency syndrome. HIV infections
Faculty: Department:	Clinical Sciences & International Health > Clinical Sciences Department
Digital Object Identifer (DOI):	https://doi.org/10.3389/fimmu.2022.992659
SWORD Depositor:	JISC Pubrouter
Depositing User:	JISC Pubrouter
Date Deposited:	14 Dec 2022 14:14
Last Modified:	13 Jun 2023 15:08
URI:	https://archive.lstmed.ac.uk/id/eprint/21259

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