Faecal volatile organic compounds differ according to inflammatory bowel disease sub-type, severity, and response to treatment in paediatric patients.

Belnour, Salma, Slater, Rachael, Tharmaratnam, Kukatharmini, Auth, Marcus Karl-Heinz, Muhammed, Rafeeq, Spray, Christine, Wang, Duolao ORCID: https://orcid.org/0000-0003-2788-2464, Ijaz, Umer Zeeshan, Probert, Chris and Allen, Stephen ORCID: https://orcid.org/0000-0001-6675-249X (2024) 'Faecal volatile organic compounds differ according to inflammatory bowel disease sub-type, severity, and response to treatment in paediatric patients.'. United European Gastroenterology Journal, Vol 12, Issue 6, pp. 780-792.

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Abstract

Background
Faecal volatile organic compounds (VOCs) differ with disease sub-type and activity in adults with established inflammatory bowel disease (IBD) taking therapy.

Objective
To describe patterns of faecal VOCs in children newly presented with IBD according to disease sub-type, severity, and response to treatment.

Methods
Children presenting with suspected IBD were recruited from three UK hospitals. Children in whom IBD was diagnosed were matched with a non-IBD child for age, sex, and recruitment site. Faecal VOCs were characterised by gas chromatography–mass spectrometry at presentation and 3 months later in children with IBD.

Results
In 132 case/control pairs, median (inter-quartile range) age in IBD was 13.3 years (10.2–14.7) and 38.6% were female. Compared with controls, the mean abundance of 27/62 (43.6%) faecal VOCs was statistically significantly decreased in Crohn's disease (CD), ulcerative colitis (UC) or both especially amongst ketones/diketones, fatty acids, and alcohols (p < 0.05). Short-chain, medium chain, and branched chain fatty acids were markedly reduced in severe colitis (p < 0.05). Despite clinical improvement in many children with IBD, the number and abundance of almost all VOCs did not increase following treatment, suggesting persistent dysbiosis. Oct-1-en-3-ol was increased in CD (p = 0.001) and UC (p = 0.012) compared with controls and decreased following treatment in UC (p = 0.01). In CD, propan-1-ol was significantly greater than controls (p < 0.001) and extensive colitis (p = 0.001) and fell with treatment (p = 0.05). Phenol was significantly greater in CD (p < 0.001) and fell with treatment in both CD (p = 0.02) and UC (p = 0.01).

Conclusion
Characterisation of faecal VOCs in an inception cohort of children with IBD reveals patterns associated with diagnosis, disease activity, and extent. Further work should investigate the relationship between VOCs and the microbiome in IBD and their role in diagnosis and disease monitoring.

Item Type:	Article
Subjects:	WI Digestive System > WI 20 Research (General) WS Pediatrics > WS 20 Research (General) WS Pediatrics > Diseases of Children and Adolescents > By System > WS 310 Digestive system
Faculty: Department:	Clinical Sciences & International Health > Clinical Sciences Department
Digital Object Identifer (DOI):	https://doi.org/10.1002/ueg2.12603
Depositing User:	Jane Rawlinson
Date Deposited:	02 Jul 2024 13:15
Last Modified:	19 Sep 2024 11:25
URI:	https://archive.lstmed.ac.uk/id/eprint/24836

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