Development of Aedes albopictus (Diptera: Culicidae) Larvae Under Different Laboratory Conditions

Puggioli, A, Carrieri, M, Dindo, ML, Medici, A, Lees, Rosemary ORCID: https://orcid.org/0000-0002-4232-9125, Gilles, JRL and Bellini, R (2017) 'Development of Aedes albopictus (Diptera: Culicidae) Larvae Under Different Laboratory Conditions'. Journal of Medical Entomology, Vol 54, Issue 1, pp. 142-149.

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Abstract

Critical to successful application of the sterile insect technique against Aedes albopictus (Skuse) is the development of an efficient and standardized rearing protocol to be employed in the mass production system. In this study several life history traits of Ae. albopictus were analyzed to identify upper and lower thresholds of larval density and diet concentration. Survival to pupation, time to pupation and sex ratio were evaluated under a range of larval densities (0.5 to 5 larvae/ml) and food doses (0.05 to 1.6 mg/larva/day) using two larval diets (one locally developed; one developed by the FAO/IAEA). The larvae reared at 28°C, at a density of 2 larvae/ml and receiving a food dose equal to 0.6 mg/larva/day of a diet consisting of 50% tuna meal, 50% bovine liver powder (the FAO/IAEA diet) and, as an additive, 0.2 gr of Vitamin Mix per 100 ml of diet solution, developed in 5 days and had 90% survival to the pupal stage. With this rearing regime the male pupae production by 24 h after the onset of pupation was the highest; these pupae were ~ 94% male.

Item Type:	Article
Subjects:	QX Parasitology > Insects. Other Parasites > QX 525 Aedes QX Parasitology > Insects. Other Parasites > QX 600 Insect control. Tick control QY Clinical Pathology > QY 25 Laboratory techniques and procedure W General Medicine. Health Professions > W 20.5 Biomedical research
Faculty: Department:	Biological Sciences > Department of Tropical Disease Biology
Digital Object Identifer (DOI):	https://doi.org/10.1093/jme/tjw127
Depositing User:	Stacy Murtagh
Date Deposited:	28 Feb 2017 16:37
Last Modified:	22 Aug 2019 11:08
URI:	https://archive.lstmed.ac.uk/id/eprint/6885

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