Aflatoxin exposure in Nigerian children with severe acute malnutrition.

McMillan, Amy, Renaud, Justin B, Burgess, Kevin M N, Orimadegun, Adebola E, Akinyinka, Olusegun O, Allen, Stephen ORCID: https://orcid.org/0000-0001-6675-249X, Miller, J David, Reid, Gregor and Sumarah, Mark W (2018) 'Aflatoxin exposure in Nigerian children with severe acute malnutrition.'. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, Vol 111, pp. 356-362.

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Abstract

Aflatoxin exposure is an important public health concern in sub-Saharan Africa as well as parts of Latin America and Asia. In addition to hepatocellular carcinoma, chronic aflatoxin exposure is believed to play a role in childhood growth impairment. The most reliable biomarker of chronic aflatoxin exposure is the aflatoxin-albumin adduct, as measured by ELISA or isotope dilution mass spectrometry (IDMS). In this report, we have used high resolution LC-MS/MS with IDMS to quantitate AFB1-lysine in an extremely vulnerable population of Nigerian children suffering from severe acute malnutrition. To increase the sensitivity and reliability of the analyses, a labelled AFB1-13C615N2-lysine internal standard was synthesized. AFB1-lysine concentrations in this population ranged between 0.2 and 59.2 pg/mg albumin, with a median value of 2.6 pg/mg albumin. AFB1-lysine concentrations were significantly higher in stunted children (median = 4.6 pg/mg) compared to non-stunted (1.2 pg/mg), as well as in children with severe acute malnutrition (4.3 pg/mg) compared to controls (0.8 pg/mg). The median concentrations were also higher in children with kwashiorkor (6.3 pg/mg) compared to those suffering from marasmus (0.9 pg/mg). This is the first report of the use of high-resolution mass spectrometry to quantitate AFB1-lysine in humans.

Item Type:	Article
Subjects:	QS Anatomy > QS 4 General works. Classify here works on regional anatomy QW Microbiology and Immunology > Antigens and Antibodies. Toxins and Antitoxins > QW 630 Toxins. Antitoxins WA Public Health > WA 30 Socioeconomic factors in public health (General) WS Pediatrics > Child Care. Nutrition. Physical Examination > WS 130 In childhood
Faculty: Department:	Clinical Sciences & International Health > Clinical Sciences Department
Digital Object Identifer (DOI):	https://doi.org/10.1016/j.fct.2017.11.030
Depositing User:	Julie Franco
Date Deposited:	01 Dec 2017 16:52
Last Modified:	12 Sep 2019 13:28
URI:	https://archive.lstmed.ac.uk/id/eprint/7891

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