Functional analysis of Salmonella Typhi adaptation to survival in water.

Kingsley, Robert A, Langridge, Gemma, Smith, Sarah E, Makendi, Carine, Fookes, Maria, Wileman, Tom M, El Ghany, Moataz Abd, Keith Turner, A, Dyson, Zoe A, Sridhar, Sushmita, Pickard, Derek, Kay, Sally, Feasey, Nicholas ORCID: https://orcid.org/0000-0003-4041-1405, Wong, Vanessa, Barquist, Lars and Dougan, Gordon (2018) 'Functional analysis of Salmonella Typhi adaptation to survival in water.'. Environmental Microbiology, Vol 20, Issue 11, pp. 4079-4090.

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Abstract

Contaminated water is a major risk factor associated with the transmission of Salmonella enterica serovar Typhi (S. Typhi), the aetiological agent of human typhoid. However, little is known about how this pathogen adapts to living in the aqueous environment. We used transcriptome analysis (RNA-seq) and transposon mutagenesis (TraDIS) to characterize these adaptive changes and identify multiple genes that contribute to survival. Over half of the genes in the S. Typhi genome altered expression level within the first 24 h following transfer from broth culture to water, although relatively few did so in the first 30 min. Genes linked to central metabolism, stress associated with arrested proton motive force and respiratory chain factors changed expression levels. Additionally, motility and chemotaxis genes increased expression, consistent with a scavenging lifestyle. The viaB-associated gene tviC encoding a glcNAc epimerase that is required for Vi polysaccharide biosynthesis was, along with several other genes, shown to contribute to survival in water. Thus, we define regulatory adaptation operating in S. Typhi that facilitates survival in water.

Item Type:	Article
Additional Information:	Thematic Issue on Pathogen and drug resistance ecology
Subjects:	QW Microbiology and Immunology > Bacteria > QW 138 Enterobacteriaceae WA Public Health > Water > WA 675 Water. Water supply. Sources
Faculty: Department:	Clinical Sciences & International Health > Clinical Sciences Department
Digital Object Identifer (DOI):	https://doi.org/10.1111/1462-2920.14458
Depositing User:	Stacy Murtagh
Date Deposited:	12 Dec 2018 15:34
Last Modified:	13 Dec 2018 16:04
URI:	https://archive.lstmed.ac.uk/id/eprint/9783

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