Isolation of an antimicrobial resistant, biofilm forming, Klebsiella grimontii isolate from a re-usable water bottle.

Hubbard, Alasdair T.M ORCID: https://orcid.org/0000-0001-6668-9179, Newire, Enas, Botelho, João, Reiné, Jesús, Wright, Elli, Hutton, William and Roberts, Adam P. ORCID: https://orcid.org/0000-0002-0760-3088 (2020) 'Isolation of an antimicrobial resistant, biofilm forming, Klebsiella grimontii isolate from a re-usable water bottle.'. MicrobiologyOpen, Vol 9, Issue 6, pp. 1128-1134.

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Abstract

A re-usable water bottle was swabbed as part of the citizen science project Swab and Send and a Klebsiella grimontii isolate was recovered on chromogenic agar and designated SS141. Whole genome sequencing of SS141 showed it has the potential to be a human pathogen as it contains the biosynthetic gene cluster for the potent cytotoxin, kleboxymycin, and genes for other virulence factors. The genome also contains the antibiotic resistance genes blaOXY-6-4 and a variant of fosA which is likely to explain the observed resistance to ampicillin, amoxicillin and fosfomycin. We have also shown that SS141 forms biofilms on both polystyrene and polypropylene surfaces, providing a reasonable explanation for its ability to colonise a re-usable water bottle. With the increasing use of re-usable water bottles as an alternative to disposables, and a strong forecast for growth in this industry over the next decade, this study highlights the need for cleanliness comparable to other re-usable culinary items.

Item Type:	Article
Subjects:	QW Microbiology and Immunology > Bacteria > QW 138 Enterobacteriaceae QW Microbiology and Immunology > QW 45 Microbial drug resistance. General or not elsewhere classified. WA Public Health > Water > WA 675 Water. Water supply. Sources WC Communicable Diseases > Infection. Bacterial Infections > Enteric Infections > WC 260 Enterobacteriaceae and other enteric infections
Faculty: Department:	Biological Sciences > Department of Tropical Disease Biology
Digital Object Identifer (DOI):	https://doi.org/10.1002/mbo3.1023
Depositing User:	Cathy Waldron
Date Deposited:	04 Mar 2020 11:18
Last Modified:	22 Jul 2020 11:48
URI:	https://archive.lstmed.ac.uk/id/eprint/13791

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